• ❖  Easy PCR kit for Mungbean yellow mosaic India virus (MYMIV)
• ❖  Easy PCR kit for Mungbean yellow mosaic virus (MYMV)
• ❖  Easy PCR kit for common detection of MYMIV and MYMV

Yellow mosaic disease is the most important production constraints of grain legumes (mungbean, blackgram, cowpea, soybean) in India. The predominant begomovirus species that infect these crops are mungbean yellow mosaic India virus (MYMIV) and mungbean yellow mosaic virus (MYMV). Close similarity in the genome of these begomoviruses makes it difficult for developing species specific diagnostics. To overcome this a novel strategy was employed to design species specific primers against MYMIV and MYMV and also a common primer set that can detect either of the begomovirus species. Using these primers, three easy PCR based diagnostic kits have been developed.

Each kit is sufficient for 50 reactions and provided with a manual. User needs to isolate DNA and follow the PCR as per the manual. A positive control and a negative control have also been included with each of the kit. After PCR if the test samples yielded fragment similar to the size of the positive control, then it can be ascertained that the sample is infected with a particular species of begomovirus. For a generic detection of either of the begomovirus species, the common easy PCR kit can be used.