The Herbarium Cryptogamae Indiae Orientalis (HCIO), a National herbarium established in 1905, not only serves as an educational resource for the National Agricultural Research System (NARS), but also conserves fungal biodiversity. At present, HCIO is enriched with nearly 51,000 specimens with Type Specimens (3800), New species records (570), New Indian Genera recorded (19), Indian Exsiccati sets (18) and Foreign Exsiccati sets (188) which are being maintained from 1892 onwards. For the researchers, students and others working in the field of Mycology and Plant Pathology, it is not always possible to visit HCIO and see the precious disease specimens. Therefore, an attempt has been made to develop a digital version of diseased specimens deposited in HCIO by various workers. The species have been arranged in alphabetical order. The specimen data in this checklist includes name of the fungus, the host or substrate, date of collection, name of the collector and a unique HCIO accession number of the specimen with a digital picture of HCIO specimen. We are very sure that, this compilation will serve as useful source to the researchers for the identification as well as recording of newer fungal species. The authors are thankful to Dr. A.K. Singh, the Director, IARI, New Delhi for providing necessary facilities to maintain and conserve the fungal biodiversity in natural and digital form. Authors are also thankful to Dr. Amrender Kumar, head, AKMU, ICAR-IARI, New Delhi for managing the database and uploaded on IARI website. The digital form of Herbarium specimens will render a wonderful facility to researchers for explore the vast collection of HCIO at their end. Authors are also thankful to Indian Council of Agricultural Research for financial support.